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Protein’s role in determining equine sex ratios unearthed

Surprisingly, many mammals do not produce equal numbers of male and female offspring.

Surprisingly, many mammals do not produce equal numbers of male and female offspring.

An Austrian study of equine embryos has unearthed strong evidence pointing to the role of insulin-like growth factor-1 (IGF1) in determining the sex ratio of equine.

It is well known that many mammals are able to adjust the ratio of male and female young depending on the surrounding conditions at the time of conception, but how precisely this is accomplished remains a matter for debate.

A recent study in the group of Christine Aurich at the University of Veterinary Medicine, Vienna, has provided important information on how the survival of female embryos may be enhanced under conditions that would otherwise tend to favour the birth of males.

Photomicrographs of embryonic tissue collected from mares at Day 12 of pregnancy. (A) Positive insulin like growth factor (IGF)-1 staining in a male embryo at Day 12. (B) Positive IGF-1 staining in a female embryo at Day 12. (C) Negative control (embryo Day 12) for secondary rabbit antibody. (D) Positive control of IGF-1 staining of equine allantochorion. Scale bars of all photomicrographs = 50 μm. .

Photomicrographs of embryonic tissue collected from mares at Day 12 of pregnancy. (A) Positive insulin like growth factor (IGF)-1 staining in a male embryo at Day 12. (B) Positive IGF-1 staining in a female embryo at Day 12. (C) Negative control (embryo Day 12) for secondary rabbit antibody. (D) Positive control of IGF-1 staining of equine allantochorion. Scale bars of all photomicrographs = 50 μm. © Elsevier

The work is published in the journal “Theriogenology”.

Because of the process involved in the formation of sperm cells, there should be an equal chance that a mammalian egg will be fertilized by “male” sperm, carrying a Y chromosome, as by a “female” sperm, carrying an X chromosome.

The symmetry of the system ensures that roughly the same number of males and females are born, which is clearly helpful for the species’ long-term survival.

Surprisingly, though, many mammals do not produce equal numbers of male and female offspring.

The discrepancy could theoretically be explained by differential fertilization efficiencies of male and female sperm (Y chromosomes are smaller than X chromosomes so perhaps male sperm can swim faster?) or by different rates of survival of male and female foetuses in the uterus.

Indeed, it does seem as though male embryos are better able to survive under conditions of high energy intake. But how does this work?

Jana Beckelmann, in Aurich’s laboratory, has unearthed provocative evidence that a particular protein, insulin-like growth factor-1, or IGF1, might somehow be involved.

From an examination of about 30 equine embryos, Beckelmann noticed that during early pregnancy (between eight and 12 days after fertilization) the level of messenger RNA encoding IGF1 was about twice as high in female embryos as in male embryos.

The difference could relate to the fact that female embryos have two X chromosomes, which might produce more of a factor required for the expression of the IGF1 gene (which is not encoded on the X chromosome) than the single X chromosome in males is able to generate.

Relative gene expression of insulin like growth factor-1 in relation to β-actin in male (gray columns) and female (hatched columns) equine embryos on Days 8 (five male, one female), 10 (five male, three female), and 12 (three male, 11 female) of pregnancy (effect of sex: P = 0.01; effect of Day: not significant; interaction sex × Day: not significant).

Relative gene expression of insulin like growth factor-1 in relation to β-actin in male (gray columns) and female (hatched columns) equine embryos on Days 8 (five male, one female), 10 (five male, three female), and 12 (three male, 11 female) of pregnancy (effect of sex: P = 0.01; effect of Day: not significant; interaction sex × Day: not significant). © Elsevier

Beckelmann was also able to confirm that the IGF1 protein was present in the embryos, confirming that the messenger RNA is actually translated to protein.

IGF1 is known to have important functions in growth and to inhibit apoptosis, or programmed cell death. As IGF1 treatment of cattle embryos produced in vivo improves their survival, it is likely that the factor has positive effects on the development of the early embryo in the horse.

So why should female embryos contain more of the factor than males?

Losses in early pregnancy are unusually high in the horse and it is believed that female embryos are especially prone to spontaneous abortion.

Male embryos are known to be better able to survive under high glucose concentrations, so well-nourished mares preferentially give birth to male foals.

“We think the higher IGF1 concentrations in female embryos might represent a mechanism to ensure the survival of the embryos under conditions that would otherwise strongly favour males,” Beckelmann says.

If this is so, the ratio of the sexes in horses is the result of a subtle interplay between environmental and internal factors, including insulin-like growth factor-1.

The paper “Sex-dependent insulin like growth factor-1 expression in preattachment equine embryos”, by Beckelmann, Sven Budik, Magdalena Helmreich, Franziska Palm, Ingrid Walter and Aurich, is published online in the journal “Theriogenology”. It will be published in print in the journal’s January 1, 2013 issue (Volume 79, Issue 1, 1 January 2013, pp. 193-199).

 

The abstract can be found at http://dx.doi.org/10.1016/j.theriogenology.2012.10.004

 

 

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